Heating new AAS?

[Beast]

Is it necessary to boil or heat up new vials of test that you receive? I've read that some people do that just to make sure it's pure of bacteria, etc.

It's also a good idea to heat it up before injecting for analgesic purposes, correct?

[restless]

You'll need a lot of dry heat in order to sterilize a sealed vial and there's really no guarantee that the hormone will survive this.

If you're really concerned about sterility buy a few sterile vials and a 0.2 microns syringe filter and filter it into the sterile vial. This will result in a technicaly sterile solution.

[Severed Ties]

I never believed that heating AS did a dam thing as far as sterility and I still don't.


ST

[gino]

Bacteria that can't survive excessive heat, don't. If you're heating the solution to 98 degrees, I can see how it could be pointless...

[BigRox]

Throw it in the oven at 400 degrees for about 10 minutes

[restless]

Bacteria that can't survive excessive heat, don't. If you're heating the solution to 98 degrees, I can see how it could be pointless...

But can you see how there's no guarantee the hormone will come out fully intact?

[gino]

But can you see how there's no guarantee the hormone will come out fully intact?

To a point... Temperature and duration are both determining factors. I've been advised(by someone with professional experience) heating should be done at 150-200 degrees at 30-60 minutes. Oxidation is really the main worry. It takes a hell of a lot of heat to start disrupting carbon-carbon bonds. Add some oxygen though, and things can start to happen, which is why one must be carefull.

Any oxidation will certainly cause a darkening of the color. No darkening means it's alright. It may be tough to tell if your sauce is a shade or two darker than it was when it went into the oven, so it's best to heat one bottle and leave one out for comparison after. Side-by-side, it should be easy to tell if there has been any oxidization.

Regardless, baking your oils is not the best/only way to insure sterility. Staph spores will survive several hundred degrees for instance. The best way to insure sterility is to steril filter through a 0.2 micron filter. That is small enough to even take out staph spores. Staph spores are small enough to make it through 0.45 micron filters, but not 0.2 micron filters.

[restless]

Staph spores are small enough to make it through 0.45 micron filters, but not 0.2 micron filters.

Exactly, so why bother with something that offers no guarantees of sterilization (dry heating) instead of filtering with 0.2 micron filters?

[BigMatt]

I heard most people Heat their oil so its lesss painfull while injection ...

[gino]

Exactly, so why bother with something that offers no guarantees of sterilization (dry heating) instead of filtering with 0.2 micron filters?

I should be more specific, I don't agree that any sterilization steps should be taken with "brand-name" goods from reputable laboratories. The vials they come in should be sterile, and taking any solution from a vial to run through a filter would be insane, and only increase the possibility of contamination. As far as biological contamination goes, the rule of thumb is obviously the fewer substance transfers, the better.

However, there are other bacterias that could be found in a home-brewed solution which live at the room-temp to body-temp range that cannot live at higher temperatures, the names of which I do not have but I remember there were at least 2...not sure if these guys can make it through a filter either, so I'd rather be safe than sorry. I'd recommend filtering and then heating(in that order, as not to damage filter) for home-brewed recipes.

I trust pharmaceutical companies to use at least more sanitary practices(I hope) than one can use in their own kitchen.

[restless]

To a point... Temperature and duration are both determining factors. I've been advised(by someone with professional experience) heating should be done at 150-200 degrees at 30-60 minutes.


Is this 150-200 F? Most sites I can find of any credibility state that around 320 F for 2 hours is what's needed to sterilize something with dry heat. It will vary with the type of material, the dimensions, heat absorption, etc...

On a related note, someone presented this to me a few days ago:

http://www.aapspharmsci.org/view.asp?art=ps050208

It seems that even 0.9 % of benzyl alcohol was enough to completely destroy all the bacteria and fungi in a multidose intravenous formulation that was on purpose contaminated with micro-organisms. The samples were even left at incubation and there were no micro-organisms at all in the benzyl alcohol sample at all after a week (or two weeks or something).

[gino]

I'll have to get in touch with my friend and find out the name of the bacteria he was referring to. This bacteria lives at a relatively low range of temperatures, the max of which isn't much higher than body temp - low level dry heat, in this case would work. I'll have to get back to you on it...

[restless]

I'll have to get in touch with my friend and find out the name of the bacteria he was referring to. This bacteria lives at a relatively low range of temperatures, the max of which isn't much higher than body temp - low level dry heat, in this case would work. I'll have to get back to you on it...

But would this specific bacteria be able to get trough a 0.2 micron filter and survive the tipical 5% BA most use in homebrews?

The question is more if there's any need to heat at all when there are much better alternatives available and not if some bacteria will get killed or not by using temperatures below the recommended for dry heating sterilization and for shorter periods of time.

I still see no need whatsoever to do this and provided you're using a 0.2 mc filter and BA at the best it will add nothing to it.

Wouldn't you agree?

[gino]

But would this specific bacteria be able to get trough a 0.2 micron filter and survive the tipical 5% BA most use in homebrews?



These things I do not know, but I'd guess the answer is no for both, assuming the bacteria in question fit the general profile of most other bacteria. So, I'd definitely agree with you.