Results 1 to 13 of 13

Thread: eggs question

  1. #1
    Senior Member Spartacus's Avatar
    Join Date
    Feb 2003
    Location
    Chicago
    Posts
    965

    eggs question

    is the avadin deactivated if they are cooked sunny side up/over easy?

  2. #2
    confused by simplicity bradley's Avatar
    Join Date
    Aug 2002
    Location
    Alabama
    Posts
    5,467
    Yes

  3. #3
    Milk, Milk, Milk JeffWillConquer's Avatar
    Join Date
    Aug 2003
    Location
    Las Vegas
    Posts
    971
    That could be the shortest answer bradley has ever given...
    Growing
    Age: 18
    Height: 5'11

  4. #4
    $3n10r M3mb3r defcon's Avatar
    Join Date
    Jul 2003
    Location
    Canada
    Posts
    1,331
    Originally posted by JeffWillConquer
    That could be the shortest answer bradley has ever given...
    hahaha, come on bradely.. dun get that lazy, i do not believe you! i demand studies

  5. #5
    confused by simplicity bradley's Avatar
    Join Date
    Aug 2002
    Location
    Alabama
    Posts
    5,467
    Fourier-transform infrared spectroscopic studies on avidin secondary structure and complexation with biotin and biotin-lipid assemblies.

    Swamy MJ, Heimburg T, Marsh D.

    School of Chemistry, University of Hyderabad, India.

    Fourier-transform infrared studies have been carried out to investigate the secondary structure and thermal stability of hen egg white avidin and its complexes with biotin and with a biotinylated lipid derivative, N-biotinyl dimyristoyl phosphatidylethanolamine (DMBPE) in aqueous dispersion. Analysis of the amide I stretching band of avidin yielded a secondary structural content composed of approximately 66% beta-sheet and extended structures, with the remainder being attributed to disordered structure and beta-turns. Binding of biotin or specific association with the biotinylated lipid DMBPE did not result in any appreciable changes in the secondary structure content of the protein, but a change in hydrogen bond stability of the beta-sheet or extended chain regions was indicated. The latter effect was enhanced by surface interactions in the case of the biotin-lipid assemblies, as was demonstrated by electrostatic binding to a nonspecific negatively charged lipid. Difference spectra of the bound biotin implicated a direct involvement of the ureido moiety in the ligand interaction that was consistent with hydrogen bonding to amino acid residues in the avidin protein. It was found that complexation with avidin leads to a decrease in bond length of the biotin ureido carbonyl group that is consistent with a reduction of sp3 character of the C-O bond when it is hydrogen bonded to the protein. Studies of the temperature dependence of the spectra revealed that for avidin alone the secondary structure was unaltered up to approximately 75 degrees C, above which the protein undergoes a highly cooperative transition to an unfolded state with concomitant loss of ordered secondary structure. The complexes of avidin with both biotin and membrane-bound DMBPE lipid assemblies display a large increase in thermal stability compared with the native protein.

  6. #6
    $3n10r M3mb3r defcon's Avatar
    Join Date
    Jul 2003
    Location
    Canada
    Posts
    1,331
    awwwwwwwwww now the thread is so much more complete, dosen't everyone agree?

  7. #7
    Milk, Milk, Milk JeffWillConquer's Avatar
    Join Date
    Aug 2003
    Location
    Las Vegas
    Posts
    971
    I agree
    Growing
    Age: 18
    Height: 5'11

  8. #8
    zen idiot Scott S's Avatar
    Join Date
    Apr 2003
    Location
    Marion, IA
    Posts
    3,205
    Yes.

  9. #9
    Newd poster SquareHead's Avatar
    Join Date
    Mar 2003
    Posts
    1,763
    HAHAHAHHAHA LMFAO
    Old Journal


    " I'd give up a pinky or a toe or something I don't need those"
    -PwrMajt:

    "Remember, as long as you don't kick them in the head or use a weapon, it's only a misdemeanor." -JustinASU

    I nominate this thread for the Tim Nissen Award! -El P

  10. #10
    100% Natural harryhoudini66's Avatar
    Join Date
    May 2003
    Location
    Los Angeles, CA
    Posts
    2,137

  11. #11
    Senior Member
    Join Date
    Jul 2003
    Posts
    3,028
    ROFL

  12. #12
    It's a Republic dammit! reloaded's Avatar
    Join Date
    Jul 2003
    Location
    Colorado
    Posts
    668
    LOL :with stupi
    Too cool for school.
    My Blog

  13. #13
    It's a Republic dammit! reloaded's Avatar
    Join Date
    Jul 2003
    Location
    Colorado
    Posts
    668
    LOL :withstupi
    Too cool for school.
    My Blog

Bookmarks

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •